Start Date: 09/02/2014
End Date: 15/02/2014
Cost: See link for details
Venue: Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
Wellcome Trust Advanced Courses
This laboratory-based advanced course will give participants a working knowledge of and practical experience in cutting edge Plasmodium experimental genetics techniques, from designing gene targeting vectors and creating transgenic parasites to phenotyping the strains that result. The goal is to facilitate the participants’ own research careers by exposing them to state-of-the-art experimental approaches, while discussing the advantages and limitations of each approach.
The week-long practical course will cover experimental approaches to both the human parasite (Plasmodium falciparum) and the rodent parasite (Plasmodium berghei). The practical work will include several novel technological approaches, including production of gene targeting constructs using recombineering, which eliminates the need for PCR and restriction enzyme based ligation, and advanced molecular phenotyping approaches including next generation RNA sequencing and comparative mass spectrometry. The laboratory-based programme will be complemented by specific lectures in topical areas as well as a panel of distinguished guest speakers, who will present the latest research in the field.