Start Date: 31/10/2016
End Date: 09/11/2016
Venue: Wellcome Genome Campus, Wellcome Genome Campus, Hinxton, CB10 1RQ, United Kingdom
Wellcome Genome Campus Advanced Courses and Scientific Conferences
Our understanding of complex genomes has benefited tremendously from whole genome sequencing projects. However, we are only beginning to systematically understand how the interplay of DNA, proteins and its biochemical modifications (i.e. chromatin) can influence global processes which control gene expression, mRNA splicing, and DNA replication or repair in normal development and in disease.
After attending the course, participants can expect to be able to:
Perform chromatin immunoprecipitation, 3C and Capture C, and DNA methylation techniques, including experimental design and end-point analysis.
Understand the methods used in the preparation of material for next generation sequencing (NGS)
Perform end-to-end analysis of NGS data, derived from the above methods, from raw sequence through to identifying relevant loci.
Download and install relevant software, and manage large datasets.
Understand how polymer physics can be used to build models of chromatin structures
Participants will receive intensive laboratory and computational training in state-of-the-art approaches focussed across five broad themes:
DNA/transcription factor interactions
Histone dynamics and modifications
Chromatin accessibility, looping and nuclear organisation
Computational analysis/integration of chromatin datasets
These sessions will be supplemented with informal tutorials and lectures from the instructors and distinguished invited speakers. Participants will analyse the various genome-wide data generated in parallel to or during the laboratory sessions. They will also have an opportunity to discuss their own research interests and present aspects of their work that are relevant to the course.