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Owing to the ongoing situation with covid-19, this event will be organised as a virtual conference. We are pleased to announce our second conference on genetic perturbations at scale. The development of CRISPR/Cas-based technologies and DNA synthesis make it possible to modulate genomes with relative ease. These tools can help us understand how genetic variation influences phenotype and thereby answer long-standing questions in biology that impact human health, laying the foundations for precision medicine for heritable diseases and cancer treatment. This rapid advancement in gene-editing technology enables us to begin to understand the functional implications of natural and disease-related human genetic variation. This year’s programme will cover approaches that modulate the genome and its context at scale, from single nucleotides and genes to hundreds of growth environments. The conference will bring together biomedical researchers working on high throughput screening, genome engineering, and/or variant effect interpretation. We welcome abstracts on all major themes of this meeting to oral or poster presentations.